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Chapters
- 00:05Title
- 01:17Overview of Stage-top Incubator for Long-term Neuronal Imaging
- 01:46Application of the Membrane Lid to the Culture Dish and Placement in the Incubator
- 02:19Live Imaging of Mitochondria in Hippocampal Neurons
- 03:44The Effects of Neuromodulation on Mitochondrial Movement in Cultured Hippocampal Neurons
- 04:27Conclusion
We describe a protocol that allows imaging of mitochondria in living neurons via fluorescence microscopy over long durations. Imaging over extended periods is accomplished through lentivirus-mediated expression of a mitochondrially targeted fluorescent protein and use of an inexpensive stage-top incubator that was designed and built in our laboratory.
Tags
NeuromodulationMitochondrial TransportLive ImagingHippocampal NeuronsExtended DurationsVital DyesFluorescent ProteinsCytoskeletal ComponentsOrganellesDynamic Fluorescence MicroscopyRhodamine 123Mitochondrially Targeted EYFPPrimary NeuronsCulture ConditionsTemperatureHumidityPHFluorescent SignalSignal IntensityImage AnalysisExposure TimesPhotobleachingPhototoxicity
