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神经调节和线粒体运输:在较长的时间里,海马神经元的实时成像
JoVE 신문
신경과학
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JoVE 신문 신경과학
Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
DOI:

04:50 min

June 17, 2011

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Chapters

  • 00:05Title
  • 01:17Overview of Stage-top Incubator for Long-term Neuronal Imaging
  • 01:46Application of the Membrane Lid to the Culture Dish and Placement in the Incubator
  • 02:19Live Imaging of Mitochondria in Hippocampal Neurons
  • 03:44The Effects of Neuromodulation on Mitochondrial Movement in Cultured Hippocampal Neurons
  • 04:27Conclusion

Summary

자동 번역

我们描述了一个协议,允许在较长的时间,通过荧光显微镜观察生活中的神经元线粒体成像。过长时间的成像是通过一个mitochondrially有针对性的荧光蛋白,并利用一种廉价的舞台上方的孵化器,在我们的实验室设计和内置的慢病毒介导的表达。

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