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传代人类神经干细胞
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Passaging Human Neural Stem Cells
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传代人类神经干细胞

DOI:
10.3791/263-v

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10:16 min

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August 22, 2007

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,
1Department of Pathology,University of California, Irvine (UCI)

Chapters

  • 00:02Title
  • 00:07Introduction
  • 01:42Preparing the Coated Flask and Dissociating the Cells
  • 06:14Centrifuging and Resuspending Cells
  • 09:44Counting Cells in Suspension

Summary

자동 번역

자동 번역

操作能力在体外培养人类神经干/前体细胞（hNSPCs）允许调查作为细胞移植用于治疗目的的实用价值与探索人类的神经系统发育。该协议提出希望增加人类干细胞研究的可重复性的培养和传代hNSPCs的方法。

Tags

PassagingHuman Neural Stem CellsHNSPCsIn VitroCell TransplantsTherapeutic PurposesHuman Neural DevelopmentPathologyCulturingStandardizing TechniqueReproducibilityStem Cell ResearchCadaveric Postnatal Brain CorticesNational Human Neural Stem Cell ResourceAdherent CulturesFibronectin CoatingDMEM:F12 Serum-free MediaEGFFGFPDGFBipolar MorphologyUndifferentiated Neural Stem CellsNestinSox2

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