Transcriptome Analysis of Single Cells

Jacqueline Morris; Jennifer M. Singh; James H. Eberwine

doi:10.3791/2634

Journal

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신경과학

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Transcriptome Analysis of Single Cells

JoVE 신문
신경과학

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JoVE 신문 신경과학

Transcriptome Analysis of Single Cells

DOI:

10.3791/2634-v

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07:27 min

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April 25, 2011

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Jacqueline Morris*¹, Jennifer M. Singh*¹, James H. Eberwine²

¹Department of Pharmacology,University of Pennsylvania, ²The Penn Genome Frontiers Institute,University of Pennsylvania

Chapters

00:05Title
01:11Preparing Culture and Pipettes
01:53Harvesting Cells
05:04Saving the Cell
06:07Representative Results for Single Cell Harvest and aRNA Analysis
07:08Conclusion

Summary

자동 번역

In this article we describe a simple method for the harvesting of single cells from rat primary neuronal cultures and subsequent transcriptome analysis using aRNA amplification. This approach is generalizable to any cell type.

Transcriptome Analysis of Single Cells

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Chapters

Summary

자동 번역

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