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Chapters
- 00:05Title
- 01:19UV Cross-linking of Tissue Culture Cells
- 02:10Bead Preparation
- 02:53Cell Lysis and Partial RNA Digestion
- 03:47Immunoprecipitation
- 04:26RNA 5′ End Labelling
- 05:08SDS-PAGE and Membrane Transfer
- 06:27Gel Purification of cDNA
- 08:44Representative Results
- 10:00Conclusion
Transkript RNA bağlayıcı proteinlerin mekansal düzenleme, post-transkripsiyonel regülasyonunda önemli bir belirleyicisidir. Bu nedenle, bir RNA bağlayıcı protein bağlama bölgeleri hassas genom haritalama sağlar bireysel nükleotid çözünürlük UV crosslinking ve immunoprecipitation (iCLIP) geliştirdi.
Tags
ICLIPTranscriptome-wide MappingProtein-RNA InteractionsIndividual Nucleotide ResolutionRNA-binding ProteinsPost-transcriptional RegulationPositional InformationBinding SitesBiochemical MethodsNative Cellular ContextAffinity PurificationImmunoprecipitationDifferential DisplayMicroarray AnalysisRIP-CHIPIndirect InteractionsNon-physiological InteractionsCLIP (UV Cross-linking And Immunoprecipitation)UV Cross-linkingRigorous Purification SchemesDenaturing Polyacrylamide Gel ElectrophoresisHigh-throughput Sequencing TechnologiesHITS-CLIPCLIP-seqPAR-CLIPPhotoreactive Ribonucleoside Analogs
