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Chapters
- 00:05Title
- 01:29Collection of Whole Blood
- 02:02Preparation of Whole Blood
- 03:53Stimulation of the Whole Blood
- 05:15Cytokine Assay
- 05:34Over-Production of IL-1B in Whole Blood from Patients with Autoinflammatory Manifestations in Response to Stimulation with LPS in the Presence of ATP
- 06:08Conclusion
We describe a simple immunoassay to measure the production of pro-inflammatory cytokines, such as IL-1 beta production, in patients presenting with autoinflammatory phenotypes. By activating cells in whole blood cultures with pathogen-associated molecular patterns, specifically with lipopolysaccharide, cytokine secretion can be conveniently evaluated in whole blood supernatants.
