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天堂斑马鱼的细胞标记与激光Uncagable荧光葡聚糖
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Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
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天堂斑马鱼的细胞标记与激光Uncagable荧光葡聚糖

DOI:
10.3791/2672-v

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07:35 min

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April 28, 2011

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, , ,
1Department of Biological Sciences,Vanderbilt University, 2Department of Chemical and Systems Biology,Stanford University

Chapters

  • 00:05Title
  • 01:05Injection of Caged Fluorescein Dextran into Zebrafish Embryos
  • 02:49Laser Uncaging of Fluorescein Dextran
  • 04:14Antibody Labeling and Detection of Uncaged Fluorescein Dextran
  • 06:36Representative Immunolabeling Results
  • 07:19Conclusion

Summary

자동 번역

자동 번역

该协议描绘的方式，标签和跟踪的小斑马鱼的胚胎细胞群体，使用紫外线uncaging笼荧光，整个装载免疫标记uncaged荧光信号放大的命运。

Tags

Lineage LabelingZebrafish CellsLaser Uncagable Fluorescein Dextran발생학Cell TypesLineage Labeling MethodsDiI LabelingTraceable EnzymesZebrafish LabsPhotoconvertable Fluorescent ProteinsEosKaedeUltraviolet LightTransgenesisDye-mediated Lineage Labeling Methods

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