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Induktion von Alloantigen-spezifische Anergie in humanen peripheren mononukleären Zellen durch Alloantigen Stimulation mit Co-stimulatorische Signal Blockade
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Chapters
- 00:05Title
- 02:00Preparation of PBMC and Bulk Alloanergizing Cultures
- 03:57Measuring the Efficacy of Co-Stimulatory Blockade and Alloanergizing by Mixed Lymphocyte Reaction
- 06:48Measuring the Specificity of Alloanergizing
- 09:05Measuring Proliferation in the Primary and Secondary MLRs by Thymidine Incorporation
- 09:43Results: Co-Stimulatory Blockade Reduces Proliferation in Primary MLR, and Alloanergizing PBMC Exhibit Decreased Proliferation in the Secondary MLR
- 10:34Conclusion
Dieses Papier beschreibt eine einfache Technik, um Alloantigen-spezifische Anergie in humanen peripheren mononukleären Zellen zu induzieren. Die Technik kann klinisch angewendet werden, um nicht alloreaktive Spenderzellen zu generieren. Infusion dieser Zellen verbessern könnte Immunrekonstitution und reduzieren Toxizität nach allogener Stammzelltransplantation.
Tags
Alloantigen-specific AnergyInductionHuman Peripheral Blood Mononuclear CellsAlloantigen StimulationCo-stimulatory Signal BlockadeAllogeneic Hematopoietic Stem Cell TransplantationGraft-versus-Host DiseaseAHSCTDonor T CellsTransplantationImmune ReconstitutionRelapse PreventionNon-specific DepletionPharmacological ImmunosuppressionAllodepletion StrategiesRecipient Antigen Presenting CellsAllodepletion TechniquesOff-target EffectsInactivation
