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Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
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Chapters
- 00:05Title
- 02:00Preparation of PBMC and Bulk Alloanergizing Cultures
- 03:57Measuring the Efficacy of Co-Stimulatory Blockade and Alloanergizing by Mixed Lymphocyte Reaction
- 06:48Measuring the Specificity of Alloanergizing
- 09:05Measuring Proliferation in the Primary and Secondary MLRs by Thymidine Incorporation
- 09:43Results: Co-Stimulatory Blockade Reduces Proliferation in Primary MLR, and Alloanergizing PBMC Exhibit Decreased Proliferation in the Secondary MLR
- 10:34Conclusion
This paper describes a simple technique to induce alloantigen-specific anergy in human peripheral blood mononuclear cells. The technique can be applied clinically to generate non-alloreactive donor cells. Infusion of these cells could improve immune reconstitution and reduce toxicity after allogeneic hematopoietic stem cell transplantation.
Tags
Alloantigen-specific AnergyInductionHuman Peripheral Blood Mononuclear CellsAlloantigen StimulationCo-stimulatory Signal BlockadeAllogeneic Hematopoietic Stem Cell TransplantationGraft-versus-Host DiseaseAHSCTDonor T CellsTransplantationImmune ReconstitutionRelapse PreventionNon-specific DepletionPharmacological ImmunosuppressionAllodepletion StrategiesRecipient Antigen Presenting CellsAllodepletion TechniquesOff-target EffectsInactivation
