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Induktion av Alloantigen-specifika anergi på humana perifera mononukleära blodceller genom Alloantigen stimulering med Co-stimulerande signal Blockade
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Chapters
- 00:05Title
- 02:00Preparation of PBMC and Bulk Alloanergizing Cultures
- 03:57Measuring the Efficacy of Co-Stimulatory Blockade and Alloanergizing by Mixed Lymphocyte Reaction
- 06:48Measuring the Specificity of Alloanergizing
- 09:05Measuring Proliferation in the Primary and Secondary MLRs by Thymidine Incorporation
- 09:43Results: Co-Stimulatory Blockade Reduces Proliferation in Primary MLR, and Alloanergizing PBMC Exhibit Decreased Proliferation in the Secondary MLR
- 10:34Conclusion
Detta dokument beskriver en enkel teknik för att inducera alloantigen-specifika anergi i humana perifera mononukleära blodceller. Tekniken kan användas kliniskt för att generera icke-alloreactive donator celler. Infusion av dessa celler skulle kunna förbättra immunförsvaret beredning och minska toxicitet efter allogen hematopoetisk stamcellstransplantation.
Tags
Alloantigen-specific AnergyInductionHuman Peripheral Blood Mononuclear CellsAlloantigen StimulationCo-stimulatory Signal BlockadeAllogeneic Hematopoietic Stem Cell TransplantationGraft-versus-Host DiseaseAHSCTDonor T CellsTransplantationImmune ReconstitutionRelapse PreventionNon-specific DepletionPharmacological ImmunosuppressionAllodepletion StrategiesRecipient Antigen Presenting CellsAllodepletion TechniquesOff-target EffectsInactivation
