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Biology

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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
 
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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Article DOI: 10.3791/2677-v 11:03 min April 29th, 2011
April 29th, 2011

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We demonstrate a chromatin immunoprecipitation (ChIP) method to identify factor interactions at tissue-specific genes during or after the onset of tissue-specific gene expression in mouse embryonic tissue. This protocol should be widely applicable for the study of tissue-specific gene activation as it occurs during normal embryonic development.

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Chromatin Immunoprecipitation Assay Tissue-specific Genes Early-stage Mouse Embryos ChIP Protein:chromatin Interactions Living Cells Tissue Culture Cells Primary Tissue Rodent Embryonic Tissue Embryonic Day 8.5 (E8.5) Embryo Chromatin Shearing Controls Specific Protein:chromatin Interactions Tissue-specific Gene Expression Programs
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