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Chapters
- 00:05Title
- 01:58Egg Collection and Dechorionation
- 02:34Microinjection with GDP-FucAI
- 03:30BTTES-Cu(I)-catalyzed Click Chemistry Reaction
- 04:34Imaging
- 05:06In Vivo Imaging of Fucosylated Glycans During Zebrafish Embryogenesis
- 06:11Conclusion
Tags
GlycansZebrafish EmbryosMetabolic LabelingBioorthogonal Click ChemistryChemical Reporter StrategyAzide-tagged MonosaccharidesAlkyne-tagged MonosaccharidesGlycan Biosynthetic MachineryCell Surface GlycoconjugatesFluorescent ProbesAffinity ProbesNoninvasive ImagingFucosylated GlycansMicroinjectionGDP-5-alkynylfucose (GDP-FucAl)Azide-conjugated FluorophoresCu(I)-catalyzed Azide-alkyne Cycloaddition (CuAAC)Confocal MicroscopySialic Acid-containing GlycansN-acetylgalactosamine-containing Glycans
