This content is Free Access.
1Department of Electrical and Computer Engineering,Boston University , 2Department of Biomedical Engineering,Boston University , 3Center for Advanced Genomics Technology,Boston University , 4Department of Medicine, Section of Infectious Diseases,Boston University School of Medicine, 5Department of Microbiology,Boston University School of Medicine, 6CNR (National Research Council),Istituto di Chimica del Riconoscimento Molecolare
Chapters
- 00:05Title
- 01:50Substrate Preparation
- 02:48Preparation of the Probe Array
- 04:50Data Acquisition and Incubation Procedure
- 07:19Data Analysis
- 08:09Representative IRIS Results
- 10:21Conclusion
Detecção biomolecular quantitativos, de alto rendimento, em tempo real, sem rótulo e (DNA, proteínas, etc) em SiO<sub> 2</sub> Superfícies pode ser conseguido usando uma técnica simples interferométricos que depende de iluminação LED, o mínimo de componentes ópticos, e uma câmera. O sensor de imagem Interferometric Reflectância (IRIS) é barato, simples de usar, e passíveis de microarray formatos.
Tags
Biomolecular DetectionInterferometric Reflectance Imaging Sensor (IRIS)Biomolecular InteractionsDiagnostic ApplicationsProteomic BiomarkersGenomic BiomarkersMolecular AffinitiesMolecular SpecificitiesLabelsImmunoassaysEnzyme-linked Immunosorbent Assay (ELISA)Polymerase Chain Reaction (PCR)Gel Electrophoresis AssaysMass Spectrometry (MS)Label-free Methods
