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1Department of Electrical and Computer Engineering,Boston University , 2Department of Biomedical Engineering,Boston University , 3Center for Advanced Genomics Technology,Boston University , 4Department of Medicine, Section of Infectious Diseases,Boston University School of Medicine, 5Department of Microbiology,Boston University School of Medicine, 6CNR (National Research Council),Istituto di Chimica del Riconoscimento Molecolare
Chapters
- 00:05Title
- 01:50Substrate Preparation
- 02:48Preparation of the Probe Array
- 04:50Data Acquisition and Incubation Procedure
- 07:19Data Analysis
- 08:09Representative IRIS Results
- 10:21Conclusion
Cuantitativo, de alto rendimiento, en tiempo real, y sin etiquetas de detección biomolecular (ADN, proteínas, etc) sobre SiO<sub> 2</sub> Superficies se puede lograr utilizando una técnica sencilla de interferometría, que se basa en la iluminación LED, un mínimo de componentes ópticos, y una cámara. El sensor de reflectancia de imágenes interferométricas (IRIS) es barato, fácil de usar, y es susceptible a los microarrays de formatos.
Tags
Biomolecular DetectionInterferometric Reflectance Imaging Sensor (IRIS)Biomolecular InteractionsDiagnostic ApplicationsProteomic BiomarkersGenomic BiomarkersMolecular AffinitiesMolecular SpecificitiesLabelsImmunoassaysEnzyme-linked Immunosorbent Assay (ELISA)Polymerase Chain Reaction (PCR)Gel Electrophoresis AssaysMass Spectrometry (MS)Label-free Methods
