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Neuroscience

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JoVE Journal Neuroscience
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
 
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Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons

Article DOI: 10.3791/2704-v • 09:56 min • May 23rd, 2011
May 23rd, 2011 •
1, 1
1Department of Molecular Pharmacology and Experimental Therapeutics, Loyola University Chicago

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We demonstrate application of the fluorescence indicator, TMRM, in cortical neurons to determine the relative changes in TMRM fluorescence intensity before and after application of a specific stimulus. We also show application of the fluorescence probe H2DCF-DA to assess the relative level of reactive oxygen species in cortical neurons.

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Mitochondrial Membrane Potential Reactive Oxygen Species Live Rat Cortical Neurons Respiratory Chain ATP Generation Oxidative Stress Environmental Toxins Oxidative Phosphorylation Mitochondrial Dysfunction Fluorescent Probes Rhodamine 123 TMRM TMRE JC-1 Fluorescence Indicators Dihydroethidium Dihydrorhodamine 123 H2DCF-DA Fluorescence Intensity
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