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JoVE Journal
Neuroscience

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In vivo Laser Axotomy in C. elegans
 
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In vivo Laser Axotomy in C. elegans

Article DOI: 10.3791/2707-v 09:19 min May 19th, 2011
May 19th, 2011

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요약

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A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.

Tags

In Vivo Laser Axotomy C. Elegans Neurons Regeneration Model Organism Genes Signaling Pathways Laser-mediated Cutting Axotomy Fluorescently-labeled Neuronal Process Femtosecond Laser Pulsed Laser MicroPoint Pulsed Laser Targeted Cell Ablation
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