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Chapters
- 00:05Title
- 00:50Introduction
- 01:09Iris Cell Culture
- 04:11Aggregation of the IPE Cells
- 05:02Implantation of Aggregated Cells and Analysis
- 06:06Role of Six-3 in Lens Regeneration by Cultured IPE Cells
- 06:41Conclusion
In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.
