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์ƒ์ฒด๊ณตํ•™
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ไธ€ไธช็ฎ€ๅ•็š„ๆ‚ฌๆปด็ป†่ƒž็”Ÿๆˆไธ‰็ปด็ƒไฝ“ๆ–‡ๅŒ–ๅ่ฎฎ
JoVE ์‹ ๋ฌธ
์ƒ์ฒด๊ณตํ•™
JoVE ๋น„๋””์˜ค๋ฅผ ํ™œ์šฉํ•˜์‹œ๋ ค๋ฉด ๋„์„œ๊ด€์„ ํ†ตํ•œ ๊ธฐ๊ด€ ๊ตฌ๋…์ด ํ•„์š”ํ•ฉ๋‹ˆ๋‹ค.  ์ „์ฒด ๋น„๋””์˜ค๋ฅผ ๋ณด์‹œ๋ ค๋ฉด ๋กœ๊ทธ์ธํ•˜๊ฑฐ๋‚˜ ๋ฌด๋ฃŒ ํŠธ๋ผ์ด์–ผ์„ ์‹œ์ž‘ํ•˜์„ธ์š”.
JoVE ์‹ ๋ฌธ ์ƒ์ฒด๊ณตํ•™
A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
Please note that all translations are automatically generated. Click here for the English version.

ไธ€ไธช็ฎ€ๅ•็š„ๆ‚ฌๆปด็ป†่ƒž็”Ÿๆˆไธ‰็ปด็ƒไฝ“ๆ–‡ๅŒ–ๅ่ฎฎ

DOI:
10.3791/2720-v

โ€ข

07:24 min

โ€ข

May 06, 2011

โ€ข

1Department of Surgery,UMDNJ-Robert Wood Johnson Medical School

Chapters

  • 00:05Title
  • 01:14Preparing a Single Cell Suspension
  • 02:57Hanging Drop Formation
  • 04:38Representative Results of Hanging Drop Cultures, Compaction of Cellular Aggregates and Spheroid Bodies
  • 07:12Conclusion

Summary

์ž๋™ ๋ฒˆ์—ญ

์ž๋™ ๋ฒˆ์—ญ

ๆˆ‘ไปฌๆ่ฟฐไบ†ไธ€ไธช็ฎ€ๅ•ๅฟซ้€Ÿ็š„ๆ–นๆณ•๏ผŒ็”Ÿๆˆไธ‰็ปด็ป„็ป‡็ฑปไผผ็š„็ƒไฝ“ๅ’Œๅ…ถๆฝœๅœจ็š„ๅบ”็”จ๏ผŒ้‡ๅŒ–๏ผŒๅœจ็ป†่ƒž้—ด็š„็›ธไบ’ไฝœ็”จ็š„ๅทฎๅผ‚ใ€‚

Tags

Hanging Drop Cell Culture3D SpheroidsCell-cell CohesionCell-substratum AdhesionMonolayer CultureRigid SubstratesIntimate ConnectionsExtracellular Matrix ComponentsChemical MilieuPhysical ForcesCellular MorphologySignalingCollagen GelsBiomaterial ScaffoldsDirect Cell-cell Adhesion ArchitectureCulture SystemsECM ProductsPhysiological ConditionsBiological Agent

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