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Chapters
- 00:05Title
- 03:22Tissue Collection and Dissociation
- 04:57Plating of Fallopian Tube Epithelial Cells
- 09:07Processing the Membrane
- 10:02Representative Results of Fallopian Tube ex vivo Cultures
- 11:24Conclusion
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
Tags
Ex Vivo CulturePrimary Human Fallopian Tube Epithelial CellsEpithelial Ovarian CancerFemale Cancer MortalityUnited StatesBreast CarcinomaUterine CarcinomaOvarian Cancer Cure RatesScreening ToolsEarly Stage Disease DetectionSurgeryChemotherapyAdvanced Stage DiseaseDiffuse Abdominal InvolvementHeterogeneous DiseaseHistologic SubtypesSerous Ovarian Carcinoma (SOC)BRCA Genes MutationsExperimental ModelsCancer Cell LinesMouse ModelsInitiating Genetic EventsPathogenesis Of DiseaseFallopian Tube Origin Of SOCFallopian Tube Secretory Epithelial Cell (FTSEC)Cell Lines Culture Systems
