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Chapters
- 00:05Title
- 01:48Aggregate Formation by the Cell Sheet Method
- 05:36Preparing the Tissue Surface Tensiometer
- 07:34Aggregate Compression
- 10:10Tissue Surface Tension Results for Aggregates of Rat Prostate Fibroblasts (RPF) and Rate Prostate Smooth Muscle Cells (RPSMC)
- 12:10Conclusion
We describe a method of measuring binding energy, expressible as tissue surface tension, between cells within 3D tissue-like aggregates. Differences in tissue surface tension have been demonstrated to correlate with invasiveness of lung, muscle, and brain tumors, and are fundamental determinants of establishing spatial relationships between different cell types.
