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Bestemmelse af molekylære strukturer af HIV Konvolut glycoproteiner med Cryo-Electron Tomography og Automatiseret Sub-tomogram Gennemsnitsberegnings
This content is Free Access.
1Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute,National Institutes of Health, 2The Medical Research Council Mitochondrial Biology Unit,University of Cambridge , 3National Library of Medicine,National Institutes of Health, 4Massachusetts Institute of Technology, 5William Fremd High School, 6University of Virginia , 7Duke University , 8Yale University, 9University of Notre Dame , 10Washington University in St. Louis , 11Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 12Thomas Jefferson High School for Science and Technology
Chapters
- 00:05Title
- 01:17Prepare Vitrified Virus Sample
- 02:39Loading Samples into the Transmission Electron Microscope
- 03:37Acquiring Cryo-electron Microscopy Data
- 04:52Converting Microscopy Data into Structural Information that can be Visualized
- 06:19Representative Results and Conclusion
Protokollen beskriver en high-throughput metode til bestemmelse af strukturer af membranproteiner bruge cryo-elektron tomografi og 3D-billedbehandling. Det dækker oplysninger om prøvepræparation, dataindsamling, databehandling og fortolkning, og slutter med produktion af et repræsentativt mål for den tilgang, de HIV-1 Konvolut glycoprotein. Disse beregningsmæssige procedurer er udformet på en måde, der gør det muligt for forskere og studerende til at arbejde eksternt og bidrage til databehandling og strukturel analyse.
Tags
HIVMolecular StructuresCryo-Electron TomographyAutomated Sub-tomogram AveragingHIV Envelope GlycoproteinsCD4+ T-cellsImmune SystemAcquired Immunodeficiency Syndrome (AIDS)Spike ProteinCD4 ReceptorCo-receptorProtein InteractionsHIV Infection PathwayHIV ResearchHIV VaccineCryo-electron Tomography3D Image ProcessingViral Membrane Proteins
