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Fastsettelse av Molecular Structures av HIV Konvolutt glykoproteiner bruker Cryo-Electron Tomography og Automated Sub-tomogram gjennomsnitt
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1Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute,National Institutes of Health, 2The Medical Research Council Mitochondrial Biology Unit,University of Cambridge , 3National Library of Medicine,National Institutes of Health, 4Massachusetts Institute of Technology, 5William Fremd High School, 6University of Virginia , 7Duke University , 8Yale University, 9University of Notre Dame , 10Washington University in St. Louis , 11Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 12Thomas Jefferson High School for Science and Technology
Chapters
- 00:05Title
- 01:17Prepare Vitrified Virus Sample
- 02:39Loading Samples into the Transmission Electron Microscope
- 03:37Acquiring Cryo-electron Microscopy Data
- 04:52Converting Microscopy Data into Structural Information that can be Visualized
- 06:19Representative Results and Conclusion
Protokollen beskriver en high-throughput tilnærming til å avgjøre strukturer av membran proteiner ved hjelp av Cryo-elektron-tomografi og 3D bildebehandling. Den dekker detaljene i prøven forberedelser, datainnsamling, databehandling og tolkning, og konkluderer med produksjon av en representant mål for tilnærming, HIV-1 konvolutt glykoprotein. Disse beregningsorientert prosedyrer er utformet på en måte som gjør at forskere og studenter til å jobbe eksternt og bidra til databehandling og strukturell analyse.
Tags
HIVMolecular StructuresCryo-Electron TomographyAutomated Sub-tomogram AveragingHIV Envelope GlycoproteinsCD4+ T-cellsImmune SystemAcquired Immunodeficiency Syndrome (AIDS)Spike ProteinCD4 ReceptorCo-receptorProtein InteractionsHIV Infection PathwayHIV ResearchHIV VaccineCryo-electron Tomography3D Image ProcessingViral Membrane Proteins
