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在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母
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A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
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在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母

DOI:
10.3791/2779-v

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10:27 min

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July 18, 2011

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, ,
1Department of Biochemistry and Molecular Biology, School of Biomedical Sciences,Monash University

Chapters

  • 00:05Title
  • 01:33Preparation of Yeast Strains
  • 03:00Cell Growth and Induction of Mitophagy by Nitrogen Starvation
  • 04:48Labeling of the Vacuole with CMAC-Arg
  • 06:26Mounting Live Yeast Cells for Fluorescence Microscopy
  • 07:52Visualizing Mitophagy by Fluorescence Microscopy
  • 10:02Conclusion

Summary

자동 번역

자동 번역

一个强大的方法来监视传递到细胞器的降解和回收的酵母液泡酸性流明描述。该方法依赖一种基因编码的双发射荧光pH值传感器，和可视化的单个细胞，用荧光显微镜对特定目标细胞器的标签。

Tags

Fluorescence MicroscopyMitophagyYeast Saccharomyces CerevisiaeAutophagyCellular TurnoverOrganelle DegradationMitochondriaHomeostasisQuality Control MechanismFluorescence ReporterRosellaPH-sensitive Green Fluorescent ProteinPH DifferencesVacuoleNitrogen Starvation

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