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Analyses quantitatives de tous influenza de type A hémagglutinines virales et les neuraminidases utilisant des anticorps Universal en simples essais slot blot
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1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB,Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products,The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 4Microbiology Department, Faculty of Medicine,King Abdulaziz University, 5National Microbiology Laboratory,Public Health Agency of Canada
Chapters
- 00:05Title
- 02:15Identification of Hemagglutinin (HA) an Neuraminidase (NA) Conserved Regions
- 03:04Slot Blot Reagent Preparation
- 03:53Preparation of Influenza Vaccine Samples and Reference Standards
- 05:20Assembly of the Bio-Dot SF Microfiltration Apparatus and Sample Blotting
- 08:08Immunoblotting of HA and NA Antigens
- 09:52Slot Blot Density Analysis
- 10:40Universal Antibodies for the Quantification of HA and NA
- 12:10Conclusion
Une méthode simple fente blot a été développé pour la quantification de l'hémagglutinine de la grippe virale et la neuraminidase utilisant des anticorps ciblant universelle leurs séquences les plus conservées identifiées par l'analyse bioinformatique. Cette approche innovatrice pourrait fournir une alternative utile pour la détermination quantitative de tous hémagglutinine et la neuraminidase virale.
Tags
Quantitative AnalysesInfluenza Type AViral HemagglutininsNeuraminidasesUniversal AntibodiesSimple Slot Blot AssaysSurface ProteinsViral Life CycleProtective Immune ResponsesNeutralizing AntibodiesInfluenza Vaccine Potency MarkerSingle Radial Immunodiffusion (SRID)Subtype-specific AntiseraNew Vaccine ReleaseNA AntigensReagentsAnalytical MethodUniversally Conserved RegionsBioinformatics AnalysesFusion PeptideEnzymatic Active SiteN-terminus
