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Kvantitativa analyser av alla influensa typ A Viral Hemagglutinins och neuroaminidaser använda Universal antikroppar i enkla mätmetoder Slot Blot
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB,Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products,The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 4Microbiology Department, Faculty of Medicine,King Abdulaziz University, 5National Microbiology Laboratory,Public Health Agency of Canada
Chapters
- 00:05Title
- 02:15Identification of Hemagglutinin (HA) an Neuraminidase (NA) Conserved Regions
- 03:04Slot Blot Reagent Preparation
- 03:53Preparation of Influenza Vaccine Samples and Reference Standards
- 05:20Assembly of the Bio-Dot SF Microfiltration Apparatus and Sample Blotting
- 08:08Immunoblotting of HA and NA Antigens
- 09:52Slot Blot Density Analysis
- 10:40Universal Antibodies for the Quantification of HA and NA
- 12:10Conclusion
En enkel slot Blot-teknik har utvecklats för kvantifiering av influensa virus hemagglutinin och neuraminidas med universella antikroppar riktade sina mest bevarade sekvenser identifierats genom bioinformatik analyser. Detta innovativa tillvägagångssätt kan ge ett användbart alternativ till kvantitativ bestämning av alla virala hemagglutinin och neuraminidas.
Tags
Quantitative AnalysesInfluenza Type AViral HemagglutininsNeuraminidasesUniversal AntibodiesSimple Slot Blot AssaysSurface ProteinsViral Life CycleProtective Immune ResponsesNeutralizing AntibodiesInfluenza Vaccine Potency MarkerSingle Radial Immunodiffusion (SRID)Subtype-specific AntiseraNew Vaccine ReleaseNA AntigensReagentsAnalytical MethodUniversally Conserved RegionsBioinformatics AnalysesFusion PeptideEnzymatic Active SiteN-terminus
