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Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
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Neuroscience
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JoVE Journal Neuroscience
Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

DOI:
10.3791/2794-v

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11:48 min

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July 13, 2011

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, ,
1Department of Physiology,Northwestern University Feinberg School of Medicine, 2Department of Psychiatry and Behavioral Sciences,Northwestern University Feinberg School of Medicine

Chapters

  • 00:05Title
  • 02:00Transfection of Primary Cultured Cortical Neurons
  • 03:14Treatment of Primary Cultured Cortical Neurons Grown on 18 mm Coverslips
  • 03:46Fixation and Immunocytohistochemistry
  • 05:21Quantitative Analysis of Spine Morphologies
  • 06:58Time-lapse Imaging
  • 09:43Representative Results: Analysis of Dendritic Spine Morphology
  • 11:04Conclusion

Summary

Automatic Translation

Automatic Translation

Numerous recent studies have identified mutations in synaptic proteins associated with brain pathologies. Primary cultured cortical neurons offer great flexibility in examining the effects of these disease-associated proteins on dendritic spine morphology and motility.

Tags

Dendritic SpinesMorphologyCultured NeuronsExcitatory ConnectionsSynapsesActinHebbian PlasticityNeuromodulatory SignalsNeural CircuitsInformation ProcessingSynaptic ProteinsDisease StatesSchizophreniaAutism Spectrum DisordersAberrant Spine PlasticityPathophysiologyCultured Cortical NeuronsHigh-resolution ImagingFixed CellsTime-lapse Imaging

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