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1Department of Medicine,Memorial Sloan Kettering Cancer Center, 2Human Oncology and Pathogenesis Program,Memorial Sloan Kettering Cancer Center, 3Developmental Biology Program,Memorial Sloan Kettering Cancer Center, 4Howard Hughes,Medical Institute, 5Laboratory of Chromatin Biology and Epigenetics,The Rockefeller University
Chapters
- 00:05Title
- 01:35Dissection of the Mouse Gastrointestinal Tract
- 02:56Whole Mount Sample Preparation and Immunostaining
- 05:10Cryosection Preparation and Immunostaining
- 07:44Representative Imaging Results
- 09:23Conclusion
The interstitial cells of Cajal (ICC) are the pacemaker cells of the gastrointestinal (GI) tract. They form complex networks between smooth muscle cells and post-ganglionic neuronal fibers to regulate GI contractility. Here, we present immunofluorescence methods cross-sectional and whole-mount visualization of murine ICC networks.
Tags
VisualizationInterstitial Cells Of Cajal (ICC)MiceGastrointestinal (GI) TractPacemaker CellsSlow WavesPeristalsisEnteric Nervous SystemMotility DisordersKIT Receptor Tyrosine KinaseKIT ImmunostainingICC NetworkKIT ActivityNeoplastic TransformationGastrointestinal Stromal Tumor (GIST)ETV1 Survival FactorICC/GIST LineageTumorigenesisActivating KIT MutationsVisualization MethodsPreviously Published ProtocolsAnoctamin 1 (ANO1)Membrane MarkerImmunofluorescence
