Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Quantification of Proteins Using Peptide…
JoVE Journal
Biology

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Biology
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
 
Click here for the English version

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Article DOI: 10.3791/2812-v • 06:09 min • July 31st, 2011
July 31st, 2011 •
*1, *1, 2, 3, 4, 5, 2, 3, 1
1Clinical Research Division, Fred Hutchinson Cancer Research Center - FHCRC, 2Department of Biochemistry and Microbiology, University of Victoria, 3Broad Institute of MIT and Harvard, 4Genome BC Proteomics Centre, University of Victoria, 5Plasma Proteome Institute
* These authors contributed equally

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) couples affinity enrichment of peptides with stable isotope dilution mass spectrometry (MRM-MS) to provide quantitative measurement of peptides as surrogates for their respective proteins. Here we describe the protocol using magnetic particles in a partially automated format.

Tags

Quantification Proteins Peptide Immunoaffinity Enrichment Mass Spectrometry Traditional Immunoassays SISCAPA Stable Isotope Standards Capture By Anti-peptide Antibodies SRM/MRM-MS Small Molecules Protein Concentration Proteotypic Peptides Trypsin
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter