High-throughput Yeast Plasmid Overexpression Screen

Michael S. Fleming; Aaron D. Gitler

doi:10.3791/2836

Journal

/

생물학

/

High-throughput Yeast Plasmid Overexpression Screen

JoVE 신문
생물학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

High-throughput Yeast Plasmid Overexpression Screen

DOI:

10.3791/2836-v

•

08:57 min

•

July 27, 2011

•

Michael S. Fleming, Aaron D. Gitler

¹Neuroscience Graduate Group,University of Pennsylvania School of Medicine , ²Department of Cell and Developmental Biology,University of Pennsylvania School of Medicine

Chapters

00:05Title
00:56Preparations for Yeast Transformation
02:19Yeast Transformation Using a Liquid Handling Robot
06:04Spotting Assay
07:10Representative Results: Plasmid Overexpression Screen
08:43Conclusion

Summary

자동 번역

Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.

High-throughput Yeast Plasmid Overexpression Screen

•

•

•

Chapters

Summary

자동 번역

Tags

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

High-throughput Saccharification Assay for Lignocellulosic Materials

High-throughput Oprensning af Affinity-mærkede rekombinante proteiner

RNA Secondary Structure Prediction Using High-throughput SHAPE

High Throughput Microinjections af søpindsvin Zygoter

High-throughput Titrering af luciferase-udtrykkende rekombinante vira

En håndbog Small Molecule Screen Nærmer High-throughput Brug zebrafisk embryoner

Inddrivelse af voksne zebrafisk Hearts for high-throughput applikationer

High-throughput screening for kemiske modulatorer af post-transkriptionelt regulerede gener

High throughput<em> Danio rerio</em> Energi Udgifter Assay

Read Article