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Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
 

Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

Article DOI: 10.3791/2855-v • 12:46 min • September 10th, 2011
September 10th, 2011 •
*1, *1, 1, 2, 1
1Biological Sciences, Kent State University, 2Marine Sciences, University of Georgia (UGA)
* These authors contributed equally

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요약
Automatic Translation

Environmental bacterioplankton are incubated with a model dissolved organic carbon (DOC) compound and a DNA labeling reagent, bromodeoxyuridine (BrdU). Afterward, DOC-degrading cells are separated from the bulk community based on their elevated BrdU incorporation using fluorescence activated cell sorting (FACS). These cells are then identified by subsequent molecular analyses.

Tags

Bromodeoxyuridine BrdU Labeling Fluorescence Activated Cell Sorting Culture-independent Identification Dissolved Organic Carbon Bacterioplankton Microbial Degradation Aquatic Environments 16S RRNA Gene-based Molecular Analysis DOC Compounds Microcosms Immunodetection HI Cells LI Cells
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