Journal
/
신경과학
/
GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
JoVE 신문
신경과학
This content is Free Access.
JoVE 신문 신경과학
GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

DOI:
10.3791/2858-v

•

09:59 min

•

July 17, 2011

•

, ,
1Department of Neuroscience,Uppsala University, Sweden

Chapters

  • 00:05Title
  • 01:42Preparation of Brain Slices
  • 03:17Preparation of Pipettes
  • 04:00Patch-clamp Experiments
  • 08:07Representative Results
  • 09:30Conclusion

Summary

자동 번역

자동 번역

We use the patch-clamp technique to measure GABA-activated single-channel currents (GABAA channels, GABAA receptors) and the synaptic and tonic currents they generate in neurons. Activation of the channels decreases neuronal excitability in health and disease 1,2,3,4.

Tags

GABA-activatedSingle-channelTonic CurrentsRat Brain SlicesGABAA ChannelsSynapsesPhasic CurrentsChloride ChannelPentameric GABA-gated ChannelChannel SubunitsFamiliesAlphasBetasFunctional ChannelSub-type SpecificNeuronal InhibitionPharmacological AgentsPatch-clamp TechniqueFunctional PropertiesHippocampal Brain SlicesSingle-channel CurrentsWhole-cell CurrentsGABA ConcentrationsDrugsIntra And Extracellular Factors

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/2858?article_type=v

Paired Whole Cell Recordings in Organotypic Hippocampal Slices
check_url/kr/2858?article_type=v

Whole-cell Patch-clamp Recordings in Brain Slices
check_url/kr/2858?article_type=v

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
check_url/kr/2858?article_type=v

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
check_url/kr/2858?article_type=v

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices
check_url/kr/2858?article_type=v

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
check_url/kr/2858?article_type=v

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices
check_url/kr/2858?article_type=v

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
check_url/kr/2858?article_type=v

Assessment of Long-term Depression Induction in Adult Cerebellar Slices
check_url/kr/2858?article_type=v

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

Read Article