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Chapters
- 00:05Title
- 02:03Site-directed Mutagenesis of Highly GC-rich KISSR Gene Sequence
- 03:48Cell Treatment and Lysis
- 06:24Immunoprecipitation of MycKISS1R
- 07:16Western Blot Detection of MycKISS1R Immunocomplexes
- 08:45Imaging and Quantification of MycKISS1R
- 10:10Analysis of KISS1R
- 11:46Conclusion
Mutations in the kisspeptin receptor (KISS1R) are associated with reproductive disorders in patients. Here we describe how to introduce mutations of interest in the GC-rich sequence of KISS1R as well as the use of KISS1R constructs to characterize the degradation pathway of the receptor by immunoprecipitation and western blot.
