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在活细胞中的荧光寿命成像分子转子
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
DOI:

09:45 min

February 09, 2012

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Chapters

  • 00:05Title
  • 01:43Staining Cells with Molecular Rotors
  • 02:57Fluorescence Lifetime Imaging Microscopy (FLIM) of Fluorescent Molecular Rotors in Cells
  • 04:50FLIM Data Analysis
  • 07:59Representative FLIM Results
  • 09:27Conclusion

Summary

자동 번역

荧光寿命成像(FLIM的)已经成为一项关键技术,对图像的环境和特定蛋白质在活细胞染料的相互作用。 FLIM的荧光分子转子允许映射在活细胞中的粘度。

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