Journal
/
면역학 및 감염병학
/
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

DOI:
10.3791/2950-v

•

09:20 min

•

October 14, 2011

•

, ,
1Institute of Plant Sciences,University of Graz, 2Institute for Electron Microscopy and Fine Structure Research,Graz University of Technology

Chapters

  • 00:05Title
  • 01:38Microwave Assisted Sample Preparation
  • 04:19Trimming and Sectioning
  • 05:36Negative Staining
  • 06:41TEM and Image Analysis
  • 07:52Representative Results of TEM
  • 08:57Conclusion

Summary

자동 번역

자동 번역

This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.

Tags

Microwave-assistedRapid DiagnosisPlant Virus DiseasesTransmission Electron MicroscopyUltrastructural ChangesSample PreparationTEM InvestigationsMicrowave FixationEmbeddingBiological SamplesTobacco Mosaic Virus (TMV)Nicotiana Tabacum PlantsUltrastructural AlterationsLeavesCommercially Available Microwave DeviceNegative StainingViral Particles

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/2950?article_type=v

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
check_url/kr/2950?article_type=v

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
check_url/kr/2950?article_type=v

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
check_url/kr/2950?article_type=v

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
check_url/kr/2950?article_type=v

High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability
check_url/kr/2950?article_type=v

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
check_url/kr/2950?article_type=v

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
check_url/kr/2950?article_type=v

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
check_url/kr/2950?article_type=v

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
check_url/kr/2950?article_type=v

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Read Article