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El aislamiento de las variantes de los virus de ARN de fidelidad y caracterización de la frecuencia de mutación de virus
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Chapters
- 00:05Title
- 01:54Determining Mutagen Toxicity for Cells
- 03:01Determining Mutagen Concentrations that Optimally Reduce Virus Titers
- 04:56Isolation and Identification of Mutagen Resistant Variants
- 06:41Confirming the Resistance Phenotype
- 08:32Checking Replication Rates and Measuring Mutation Frequencies
- 10:40Sequence Analysis
- 12:34Representative Results
- 17:27Conclusion
Tags
RNA VirusesFidelity VariantsVirus Mutation FrequencyRNA Dependent RNA PolymerasesExtreme Population DiversityVirus AdaptationVirus EvolutionIntrinsic Error RatesViral PolymeraseIncorporation FidelityMutation FrequenciesVirological TechniquesSequencing TechniquesStatistical SignificancePolymerase Structure And FunctionVirus Adaptation Mechanisms
