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Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
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Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

DOI:
10.3791/2954-v

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13:47 min

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June 23, 2011

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, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide,Beckman Research Institute of City of Hope

Chapters

  • 00:05Title
  • 01:20Preparation of RNA Library
  • 03:00In vitro Generation of Aptamers by Selex
  • 06:47Monitoring SELEX Progress
  • 09:51Isolation and Characterization of New RNA Aptamers Against HIV-1BaL gp120
  • 13:27Conclusion

Summary

자동 번역

자동 번역

Several 2’-Fluoro RNA aptamers against HIV-1Ba-L gp120 with nanomole affinity are isolated from a RNA library by in vitro SELEX procedure. A new dual inhibitory function anti-gp120 aptamer-siRNA chimera is created and shows considerable promise for systemic anti-HIV therapy.

Tags

Cell-type SpecificAnti-HIVGp120 AptamersSiRNA DeliveryHIV InfectionAntiretroviral AgentsSmall Interfering (si)RNAsTherapeuticsRNAi-basedHIV/AIDSRNA TranscriptsEfficient DeliveryTargeted Intracellular SiRNA Delivery ApproachHIV-1 Gp120 ProteinGlycoprotein EnvelopeViral EntryCD4 CellsAnti-viral StrategyDrug Discovery2′-F Modified Anti-HIV Gp120 RNA AptamersNitrocellulose Filter SELEX Method

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