JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:31Insertion of Tracheal Tube
- 02:51Immune Cell Isolation
- 05:54Representative Histological, Functional and Flow-cytometric Results of Cardiac Immune Isolated Cells
- 07:05Conclusion
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
Tags
Functional Cardiac Immune CellsPathological RemodelingCardiac DiseasesMast CellsT-cellsMacrophagesBiologically Active MediatorsCytokinesProteasesTryptaseExtracellular Matrix MetabolismMatrix MetalloproteinasesCollagen AccumulationCardiac Fibroblasts’ FunctionIsolating Cardiac Immune CellsLoss Of FunctionTechnique DevelopmentUnderstanding Of Role In Cardiac DiseaseAnatomical LocationRat’s Xiphoid ProcessAxillaFalciform LigamentPericardium Of The HeartCharacterization Of FunctionalityPhenotypeMaturity
