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Chapters
- 00:05Title
- 01:22Exosome Isolation
- 02:53Exosome Identification by Electron Microscopy
- 03:23Exosome Detection and Characterization by Flow Cytometry
- 04:49Exosome Detection and Characterization by Western Blot
- 06:08Isolation and Analysis of Exosomal RNA
- 07:13Results: Exosome Analyses
- 08:49Conclusion
This paper demonstrates methods for the isolation, purification and detection of exosomes, as well as techniques for analysis of their molecular content. These methods are adaptable for exosome isolation from both cell culture media and biological fluids, and can beyond analysis of molecular content also be useful in functional studies.
Tags
Exosome ResearchRNA-containing ExosomesVesiclesEndocytic OriginTransferrin ReceptorLate EndosomesMultivesicular Bodies (MVBs)Plasma Membrane FusionCell Release Of ExosomesBiological FluidsContent And Function Of ExosomesTolerance InductionTumor EradicationNatural Killer CellsT Regulatory CellsMRNA And MicroRNA In Exosomes
