JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 02:08Tosylactivated Bead Coating
- 04:25PBMC Isolation
- 05:41MACS Pre-sort of CD4 T Cells
- 08:05Fluorescent Activated Cell Sorting (FACS) Isolation
- 09:59Cell Culture Setup, Harvesting, and Counting
- 11:32Tregs Differ in their Ability to Successfully Suppress Naive and in vivo-activated T Cells
- 13:34Conclusion
Tags
Human In Vitro SuppressionScreening ToolRecognitionEarly StateImmune ImbalanceRegulatory T CellsTregsImmune ToleranceSelf-antigensInfectionSurface MarkerProliferationFunctionEffector T CellsIn Vitro AssaysCross-sectional StudiesAutoimmune DiseasesType 1 Diabetes-T1DLaboratoriesResponder T CellsStimulationTreg:responder RatiosAntigen-presenting Cells (APC)VariabilityComparison Between StudiesStandardized Suppression AssayHealthy SubjectsAutoimmune Destruction Of Pancreatic β-cells
