Journal
/
면역학 및 감염병학
/
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

DOI:
10.3791/3094-v

•

12:39 min

•

June 24, 2011

•

, , ,
1Department of Laboratory Medicine and Pathology,Mayo Clinic

Chapters

  • 00:05Title
  • 01:47Experimental Preparation and Biosafety Procedures
  • 03:13Preparation of the Inoculum
  • 05:05Plating the Inoculum
  • 08:07Determining Antibiotic Resistance Manually
  • 09:12Determining Antibiotic Resistance Using the Vizion Platform
  • 10:10Determining Sample Purity and Performing Colony Counts
  • 10:57Representative Antimicrobial Susceptibility Assay
  • 12:00Conclusion

Summary

자동 번역

자동 번역

Antimicrobial susceptibility testing of Mycobacterium tuberculosis is challenging but critical for patient care. This microtiter plate format offers testing of 12 antimycobacterial drugs and provides minimum inhibitory concentration (MIC) values, which will aid in appropriate treatment.

Tags

Antimicrobial Susceptibility TestingMycobacterium Tuberculosis ComplexFirst Line DrugsSecond Line DrugsBroth DilutionMicrotiter Plate FormatAgar Method Of ProportionMacrobroth TestingBACTECVersaTREKBacT/ALERTAgar Proportion MethodResistance CalculationDrug-containing AgarDrug-free AgarMacrobroth MethodsCritical Drug ConcentrationsMYCOTBTuberculosis Treatment

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/3094?article_type=v

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
check_url/kr/3094?article_type=v

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
check_url/kr/3094?article_type=v

Growth of Mycobacterium tuberculosis Biofilms
check_url/kr/3094?article_type=v

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
check_url/kr/3094?article_type=v

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
check_url/kr/3094?article_type=v

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
check_url/kr/3094?article_type=v

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
check_url/kr/3094?article_type=v

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
check_url/kr/3094?article_type=v

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates
check_url/kr/3094?article_type=v

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Read Article