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检测组蛋白修饰在植物叶片
JoVE 신문
생물학
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JoVE 신문 생물학
Detection of Histone Modifications in Plant Leaves
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检测组蛋白修饰在植物叶片

DOI:
10.3791/3096-v

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07:08 min

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September 23, 2011

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, ,
1Department of Botany,RWTH Aachen University, 2Department of Plant Physiology,RWTH Aachen University, 3Department of Botany,Leibniz University

Chapters

  • 00:05Title
  • 01:25Crosslinking of Plant Material
  • 02:25Isolation of Chromatin
  • 04:18Immunoprecipitation
  • 05:26Locus-specific DNA Quantitation
  • 05:55Benzothiadiazole (BTH) Induced Histone Acetylation on the PR2 Promoter in Arabidopsis
  • 06:20Conclusion

Summary

자동 번역

자동 번역

一个可靠的和有用的方法来检测特定的植物基因组蛋白修饰的描述。该方法结合了染色质免疫沉淀（ChIP）和实时定量PCR。它允许在不同的生理过程作用，对特定的基因组蛋白修饰的检测。

Tags

Histone ModificationsPlant LeavesChromatin StructureGene Expression RegulationChromatin RemodelingDNA MethylationCovalent ModificationsHistones H3 And H4Lysine MethylationArginine MethylationLysine AcetylationSerine PhosphorylationGene ActivationGene RepressionPrimed State Of GeneChromatin Immunoprecipitation (ChIP)Modification-specific AntibodiesReal-time Quantitative PCRC4 Photosynthesis In MaizeSystemic Immunity In Arabidopsis

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