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Chapters
- 00:05Title
- 01:25Crosslinking of Plant Material
- 02:25Isolation of Chromatin
- 04:18Immunoprecipitation
- 05:26Locus-specific DNA Quantitation
- 05:55Benzothiadiazole (BTH) Induced Histone Acetylation on the PR2 Promoter in Arabidopsis
- 06:20Conclusion
Un approccio affidabile e utile per rilevare modificazioni degli istoni sui geni impianto specifico è descritto. L'approccio combina immunoprecipitazione della cromatina (ChIP) e real-time PCR quantitativa. Esso consente il rilevamento di modifiche degli istoni su specifici geni con un ruolo in diversi processi fisiologici.
Tags
Histone ModificationsPlant LeavesChromatin StructureGene Expression RegulationChromatin RemodelingDNA MethylationCovalent ModificationsHistones H3 And H4Lysine MethylationArginine MethylationLysine AcetylationSerine PhosphorylationGene ActivationGene RepressionPrimed State Of GeneChromatin Immunoprecipitation (ChIP)Modification-specific AntibodiesReal-time Quantitative PCRC4 Photosynthesis In MaizeSystemic Immunity In Arabidopsis
