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Chapters
- 00:05Title
- 01:43Isolation of Rat Mesenteric Small Artery
- 02:45Mounting of Vessel
- 04:43Normalization
- 05:31Measurement of Responses
- 06:24Contraction and Relaxation in Mesenteric Arteries
- 07:15Conclusion
An automated myography method for force measurements in isolated mesenteric arteries is described. It employs a Mulvany-Halpern Auto Dual Wire Myograph 510A to determine responses to phenylephrine and extracellular calcium. The method allows consistent determination of isometric responses to agonists in small vessels of diameters of 60 - 300 μm, independently.
Tags
Mesenteric ArteryContractionRelaxationAutomated Wire MyographyProximal Resistance VesselsBlood FlowRat Mesenteric ArteryMyography TechniqueIsometric ConditionsForce ProductionSensitivityAgonistsStretchCompliance Of Mounting WiresStainless Steel WiresTungsten WiresOxidationRecorded ResponsesVascular Smooth Muscle Cell ReceptorsPhenylephrineExtracellular Calcium (Ca2+e)
