JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Temple University,Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service,Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy,Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology,Temple University School of Medicine
Chapters
- 00:05Title
- 01:19Preparation for Surgery
- 02:19Laminectomy and Surgical Exposure of the L5 Dorsal Root
- 03:21Rhizotomy/Dorsal Root Crush
- 04:10Image Acquisition and Post-op Procedures
- 04:51Repeated Imaging
- 05:32Axonal Regeneration Following Dorsal Root Crush
- 06:16Conclusion
En<em> In vivo</em> Bildprotokoll att övervaka primära sensoriska axoner efter dorsala krossa beskrivs. De förfaranden som utnyttjar brett fält fluorescensmikroskopi och thy1-YFP transgena möss, och tillåta upprepad avbildning av Axon förnyelse över 4 cm i PNS och interaktion axon med gränssnittet i CNS.
Tags
Live ImagingDorsal Root AxonsRhizotomySpinal Root InjuriesChronic PainSensory LossRegenerationDorsal Root Entry Zone (DREZ)Molecular EventsCellular EventsNerve InjuryPostmortem AnalysesAxon RegenerationReal-time EventsLiving AnimalNeurons Serially In VivoOpticsMouse TransgenicsThy1-GFP Transgenic MiceFluorescent ColorsIn Vivo ImagingMuscleBrainPhysiological MechanismsStatic AnalysesSpinal Cord Imaging
