JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:54Brain Slice Preparation
- 01:42Electrophysiological Fingerprinting of Nigral Neurons
- 02:48Cytoplasm Aspiration
- 03:29Reverse Transcription (RT) Reaction
- 04:23Two Stage PCR Amplification
- 05:14Electrophysiology and Gene Expression in Selected Neurons
- 07:04Conclusion
Las neuronas se caracterizan primera electrofisiológica. A continuación, el citoplasma de las neuronas registradas se aspira y se somete a la transcripción reversa-PCR y análisis para detectar la expresión de ARNm para enzimas síntesis de neurotransmisores, canales iónicos y receptores.
Tags
ProfilingVoltage-gated Potassium ChannelMRNA ExpressionNigral NeuronsSingle-cell RT-PCR TechniquesMammalian Central Nervous SystemNeuronsMolecular CharacteristicsFunctional CharacteristicsMorphologyGene Expression AnalysisSpecific Neuron TypeWhole-cell Patch Clamp Recording TechniquesSingle-cell Reverse Transcription Polymerase Chain Reaction (scRT-PCR)MRNA Expression ProfilingSubstantial NigraElectrophysiological TechniquesNeurophysiological PropertiesFunctional PropertiesCytoplasm AspirationScRT-PCR AnalysisNeurotransmitter Synthesis EnzymesReceptorsIon ChannelsSelectivitySensitivityImmunohistochemistryAntibody SuitabilityProtein Expression Level
