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分离的CD133 +肝干细胞的克隆扩增

JoVE 신문
생물학

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JoVE 신문 생물학

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

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分离的CD133 +肝干细胞的克隆扩增

DOI:

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12:06 min

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October 10, 2011

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C. Bart Rountree, Wei Ding, Hein Dang, Colleen VanKirk, Gay M. Crooks

¹Department of Pediatrics and Pharmacology,Pennsylvania State College of Medicine, ²Department of Pharmacology,Pennsylvania State College of Medicine, ³Department of Pediatrics,University of California Los Angeles, School of Medicine

Chapters

00:05Title
01:45Parenchymal and Non-parenchymal Separation from Whole Liver
04:34Red Cell Lysis
05:51CD45 Hematopoietic Cell Depletion from Non-parenchymal Fraction
07:31Flow Cytometry Isolation of CD133 Positive Cells
09:03Representative Cell Sorting and Analysis
11:14Conclusion

Summary

자동 번역

在这里，我们描述CD133的表达肝干细胞和整体的小鼠肝，一个过程，需要组织消化，细胞富集和流式细胞仪分离肿瘤干细胞的分离。包括先进的单细胞的分离和克隆扩增的方法。

Tags

CD133+ Liver Stem Cells Clonal Expansion Chronic Liver Injury Hepatocytes Cholangiocytes Liver Cancer Hepatocellular Carcinoma Stem Cell Isolation Solid Organ Rare Population Of Cells Parenchymal Fraction Non-parenchymal Fraction CD45 Negative Cells Enrichment Stem Cell Proliferation Immunohistochemistry Flow Cytometry Core

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