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Chapters
- 00:05Title
- 01:37PORN-H/Laminin Coating of Dishes and Coverslips
- 02:33Lectin Coating of the Purification Plate
- 03:20Motoneuron Culture Medium
- 04:32Spinal Cord Dissection
- 05:35Enrichment of Motoneurons by Lectin-based Purification
- 07:28Results: Lectin-based Isolation and Characterization of Mouse Embryonic Motoneurons
- 08:46Conclusion
Ett alternativt sätt att isolera möss embryonala motoneurons från ryggmärgen beskrivs. Metoden tar hänsyn till det faktum att lektin kan binda till låga affinitet nerve growth factor receptor p75NTR. Denna lektin-baserade preplating ger en reningsanläggning som liknar den som med en specifik antikropp mot p75NTR.
Tags
Lectin-based IsolationCultureMouse Embryonic MotoneuronsSpinal MotoneuronsNeuroepithelial CellsNkx6.1Precursor CellsPostmitotic StagesDendritesAxonsTranscription FactorsIslet 1/2Innervate MusclesHigh Affinity ReceptorsBrain Derived Neurotrophic Factor (BDNF)Neurotrophin-3 (NT-3)Tropomyosin-related Kinase B And C (TrkBTrkC)Tropomyosin-related Kinase A (TrkA)Low Affinity Neurotrophin Receptor P75NTRIsolation TechniquesSpecific Antibodies (panning)
