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采用TaqMan从儿童凳诺如病毒的检测和Genogrouping一步法RT-PCR技术

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Detection and Genogrouping of Noroviruses from Children’s Stools By Taqman One-step RT-PCR

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采用TaqMan从儿童凳诺如病毒的检测和Genogrouping一步法RT-PCR技术

DOI:

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15:16 min

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July 22, 2012

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Sonia Apaza, Susan Espetia, Robert H. Gilman², Sonia Montenegro, Susana Pineda, Fanny Herhold, Romeo Pomari, Margaret Kosek, Nancy Vu, Mayuko Saito^2,4

¹Laboratorio de Investigación y Desarrollo (LID),Universidad Peruana Cayetano Heredia, ²Bloomberg School of Public Health,Johns Hopkins University, ³Laboratorio de Diagnostico Molecular, Facultad de Medicina,University of Concepcion,Chile, ⁴University of California San Diego School of Medicine

Chapters

00:05Title
01:37In-house Silica-guanidinium Method
02:02Preparation of Silica Suspension for RNA Extraction
03:24Extraction with Guanidine & Silica
06:08Commercial Extraction of Viral RNA: QIAamp Viral RNA Kit
08:32Detection of NoV in Extracted RNA by One-step Real Time PCR with Specific Taqman Probes
14:11Conclusion

Summary

자동 번역

一个一步法RT-PCR方法检测检测和孩子的大便，，利用引物和的TaqMan探针的开放阅读框（ORF1基因）的病毒ORF2交界地区，对诺沃克类病毒基因组中最保守的地区是特定genogroup诺沃克病毒分离鉴定描述。详述一个非商业化，具有成本效益的RNA提取方法。

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