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Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting

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Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting

Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting

DOI:

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13:41 min

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October 17, 2011

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Ravichandra Bachu*¹, Frances-Camille S. Padlan*², Sara Rouhanifard, Michael Brenowitz, Jörg C. Schlatterer

¹Department of Chemistry,Hunter College , ²Department of Biochemistry,Albert Einstein College of Medicine

Chapters

00:05Title
02:24RNA Preparation for Footprinting Experiment
04:24The Footprinting Experiment
08:09Data Analysis
10:38Analysis of RNA Fragments after Peroxidative Fenton Footprinting Reaction
12:23Conclusion

Summary

자동 번역

This protocol describes how to quantify the Mg(II)-dependent formation of RNA tertiary structure by two methods of hydroxyl radical footprinting.

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