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收养大鼠迟发型超敏反应的感应和监测
JoVE 신문
생물학
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JoVE 신문 생물학
Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats
DOI:

22:06 min

October 01, 2007

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Chapters

  • 00:00Title
  • 00:06Introduction
  • 00:51Cardiac Puncture and Dissection of Thymus
  • 02:52Isolation of Antigen Presenting Cells (APCs) from Thymus
  • 06:24Activation of OVA-specific T cells by Thymic APCs
  • 12:55Adoptive Transfer of OVA-specific T cells
  • 19:27Challenging with OVA in the Ear
  • 21:02Measurment of Inflammation in the Ear
  • 21:27Conclusion

Summary

자동 번역

迟发型超敏反应(DTH)是CCR7的效应记忆T(TEM)淋巴细胞介导的​​炎症反应。在这里,我们将演示如何激活抗原特异性的透射电镜细胞,诱导Lewis大鼠收养的DTH和监测的炎症反应。

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